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Gataca Inc ilas2 tirf frap illumination module
Ilas2 Tirf Frap Illumination Module, supplied by Gataca Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ilas2+tirf+frap+module/frap+ilas2+module+tirf/pm41862644-319-33-32
Average 86 stars, based on 1 article reviews
ilas2 tirf frap illumination module - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

other:

Article Title: Stu2 performs an essential kinetochore function independent of its microtubule polymerase activity.
Article Snippet: ch-TOG family proteins, including the budding yeast Stu2, are essential for spindle formation and chromosome segregation.. Such functions depend on an array of activities ranging from microtubule nucleation, polymerization, and depolymerization to conferring tension sensitivity to kinetochores.. This functional diversity makes it challenging to dissect these various functions and understand their relative importance.

Microscopy:

Article Title: Mechanisms of Force Generation by Actin Filament Associated Proteins in Budding Yeast Clathrin-mediated Endocytosis
Article Snippet: Quantitative fluorescence microscopy was performed using a Nikon Ti2-E inverted microscope with an Oko Labs environmental chamber pre-warmed to 25°C. .. GFP and FM4-64 were excited by a 488-nm laser and mRFP was excited by a 561-nm laser using highly inclined and laminated optical sheet (HILO) microscopy to illuminate the medial focal plane of the sample using a LUNF 4-line laser launch (Nikon Instruments, Melville, NY) and an iLas2 TIRF/FRAP module (Gataca Systems, Massy, France). ..

Software:

Article Title: Comparative analysis reveals distinct actin-based motility mechanisms in divergent Rickettsia species
Article Snippet: Imaging was carried out on a Nikon Ti Eclipse Ti2 inverted microscope equipped with a Nikon 60x/1.49 NA CFI Apo TIRF objective and temperature chamber set to 25°C (Oko labs). .. Single-plane TIRF images were illuminated with a LUNF 4-line laser launch and iLas2 TIRF/FRAP module (Gataca Systems) and captured with an Orca Fusion Gen III sCMOS camera (Hamamatsu) every 5 s for 15-20 m with NIS Elements software (Nikon). ..

Fluorescence:

Article Title: Class-I myosin responds to changes in membrane tension during clathrin-mediated endocytosis in human induced pluripotent stem cells.
Article Snippet: Images were acquired using a Nikon 60× CFI Apo TIRF objective (1.49 NA) and an Orca Fusion Gen III sCMOS camera (Hamamatsu) at 1× magnification using Nikon NIS Elements software. .. Using a LUNF 4- line laser launch (Nikon Instruments) and iLas2 TIRF/FRAP module (Gataca Systems), total internal reflection fluorescence (TIRF) illuminated a single focal plane of the field and was imaged every second for 5 min. ..



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Danaher Inc tirf ilas2 tirf frap module
A, Workflow of condensates immobilization. Image created with BioRender.com . B, Representative <t>TIRF</t> imaging of anchor points on PF127-passivated glass incubated with varying concentrations of Biotin-BSA. Plot illustrates the density of anchor points for each concentration (mean ± SD, n>20 images for each concentration). C, Movement trajectories of polySUMO/polySIM condensates at varying biotin-BSA concentration. D, Orthogonal views of polySUMO/polySIM condensates at varying biotin-BSA concentration (note that apparently irregular shapes at low biotin-BSA concentrations are due to movement during imaging of the confocal stack). Plot illustrates contact angle of polySUMO/polySIM condensates at different biotin-BSA concentrations (median and quartiles, n>15 condensates for each condition). E, Partial FRAP of polySUMO/polySIM condensates on glass passivated by PF127 with anchor points or mPEG/BSA (mean ± SD, n=12 condensates for each condition). For comparison, partial FRAP of polySUMO/polySIM condensates on PF127-passivated glass without anchor points is displayed at a different time resolution at the bottom.
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A, Workflow of condensates immobilization. Image created with BioRender.com . B, Representative TIRF imaging of anchor points on PF127-passivated glass incubated with varying concentrations of Biotin-BSA. Plot illustrates the density of anchor points for each concentration (mean ± SD, n>20 images for each concentration). C, Movement trajectories of polySUMO/polySIM condensates at varying biotin-BSA concentration. D, Orthogonal views of polySUMO/polySIM condensates at varying biotin-BSA concentration (note that apparently irregular shapes at low biotin-BSA concentrations are due to movement during imaging of the confocal stack). Plot illustrates contact angle of polySUMO/polySIM condensates at different biotin-BSA concentrations (median and quartiles, n>15 condensates for each condition). E, Partial FRAP of polySUMO/polySIM condensates on glass passivated by PF127 with anchor points or mPEG/BSA (mean ± SD, n=12 condensates for each condition). For comparison, partial FRAP of polySUMO/polySIM condensates on PF127-passivated glass without anchor points is displayed at a different time resolution at the bottom.

Journal: bioRxiv

Article Title: Advanced Surface Passivation for High-Sensitivity Studies of Biomolecular Condensates

doi: 10.1101/2024.02.12.580000

Figure Lengend Snippet: A, Workflow of condensates immobilization. Image created with BioRender.com . B, Representative TIRF imaging of anchor points on PF127-passivated glass incubated with varying concentrations of Biotin-BSA. Plot illustrates the density of anchor points for each concentration (mean ± SD, n>20 images for each concentration). C, Movement trajectories of polySUMO/polySIM condensates at varying biotin-BSA concentration. D, Orthogonal views of polySUMO/polySIM condensates at varying biotin-BSA concentration (note that apparently irregular shapes at low biotin-BSA concentrations are due to movement during imaging of the confocal stack). Plot illustrates contact angle of polySUMO/polySIM condensates at different biotin-BSA concentrations (median and quartiles, n>15 condensates for each condition). E, Partial FRAP of polySUMO/polySIM condensates on glass passivated by PF127 with anchor points or mPEG/BSA (mean ± SD, n=12 condensates for each condition). For comparison, partial FRAP of polySUMO/polySIM condensates on PF127-passivated glass without anchor points is displayed at a different time resolution at the bottom.

Article Snippet: Total Internal Reflection Fluorescence (TIRF) images were captured using a TIRF/iLAS2 TIRF/FRAP module (Biovision) mounted on a Leica DMI6000 microscope base equipped with Leica 100× 1.49 NA oil immersion objective ( ) or a DeltaVision OMX SR system, equipped with an Olympus 60 × 1.49 NA oil immersion objective for TIRF and ring-TIRF capabilities ( ).

Techniques: Imaging, Incubation, Concentration Assay, Comparison